QUANTIFOIL® Holey Carbon Films | ||||
Descrption | QUANTIFOIL® is a perforated support foil with a pre-defined hole size, shape and arrangement. We offer a variety of QUANTIFOIL® Holey Carbon Films with orthogonally arranged circular holes that differ in hole size and bar width. We also offer QUANTIFOIL® types with square holes and hexagonal geometry.If you would like to try out different types of QUANTIFOIL® Holey Carbon Films in order to find out which one meets your requirements best, you can order our MixBox with 30 support foils that contains five QUANTIFOIL® types (R 1.2/1.3, R 2/1, R 3.5/1, S 7/2, Multi A) and the standard Carbon Support Films on 300 mesh Copper grids. | |||
QUANTIFOIL® with circular holes | ||||
Description |
QUANTIFOIL® with circular holes is mainly used in cryo-EM. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The hole size that is chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. The latter makes assessment of the image quality easier, because the power spectrum of a foil is stronger than that of unsupported ice. The QUANTIFOIL® types with small holes (e.g. 1.2 µm) were designed for magnifications around and higher 50.000x. At magnifications between 30.000x and 40.000x we recommend the QUANTIFOIL® types with 2 µm holes. Larger holes (e.g. 3.5 µm, 5 µm) are suitable at magnification around 20.000x. | |||
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Descrption | QUANTIFOIL® with square holes and relatively narrow bars can be used in electron microscopy to support a thin carbon film, which by itself is too fragile to span a grid square. The QUANTIFOIL® types with square holes constitute an optimum between a maximum of open area on the one hand, and mechanical stability on the other hand. | |||
Quantifoil Support Films for Electron Microscopy | ||||
No. | D μm | Widthμm | Periodμm | Shape |
R 0.6/1 | 0.6 | 1 | 1.6 | Hole |
R1/1 | 1 | 1 | 2 | Hole |
R1/2 | 1 | 2 | 3 | Hole |
R 1/4 | 1 | 4 | 5 | Hole |
R 1.2/1.3 | 1.2 | 1.3 | 2.5 | Hole |
R 1.2/20 | 1.2 | 20 | 21.2 | Hole |
R 2/1 | 2 | 1 | 3 | Hole |
R 2/2 | 2 | 2 | 4 | Hole |
R 2/4 | 2 | 4 | 6 | Hole |
R 3/3 | 3 | 3 | 6 | Hole |
R 3/5 | 3 | 5 | 8 | Hole |
R 3.5/1 | 3.5 | 1 | 4.5 | Hole |
R 5/10 | 5 | 10 | 15 | Hole |
R 5/20 | 5 | 20 | 25 | Hole |
R 6/6.5 | 6 | 6.5 | 12.5 | Hole |
R 6/100 | 6 | 100 | 106 | Hole |
R 10/5 | 10 | 5 | 15 | Hole |
R10/10 | 10 | 10 | 20 | Hole |
R 10/20 | 10 | 20 | 30 | Hole |
R 17/5 | 17.5 | 5 | 22.5 | Hole |
S 7/2 | 7 | 2 | 9 | Square |
S35/5 | 35 | 5 | 40 | Square |
S35/10 | 35 | 10 | 45 | Square |