The new BI-4500 surface
plasmon resonance microscopy (SPR) has multiple channel flow pattern, which
facilitates accurate detection of analytes with low fixed quantities and low
molecular weight (<100 Da). Equipped with BI-DirectFlowTM (bi-DC
technology), the BI-4500 combines precise sample delivery and nearly-zero
diffusion transfer process for rapid kinetic studies and effectively eliminates
the interference of various surface phenomena.Users have the flexibility to
choose from a variety of ingenious analysis modules for studies such as life
sciences, electrochemistry, gas phase or liquid phase sensing.Three channel
surface plasmon resonance microscopy
l High
sensitivity to measure small molecules
l High
throughput with 5 channels and fully automated sampling
l Precise
sample delivery with BI-DirectFlowTM technology
l Innovative
multi-module design for optimalflexibility
l Cost
effective solution
Function
Main function
Kinetics &
Quantitative Analysis (Ka,Kd,KD,C)
Biomarker detection,
drug targeted development
Food detection,
environmental monitoring
Small molecule
free-label analysis (<100Da)
Film thickness and
structure change on material surface
Electrochemical
simultaneous analysis
Gas molecules, sugar
molecules, DNA, antibodies, peptides, proteins, viruses, bacteria, cancer cells
Technology features
SPR
technology is an optical detection technology used to test the thickness and
structure changes of ultrathin adsorption layer on the surface of sensor chip,
which is a powerful tool to study biomolecules and their interactions with
other substances.
High sensitivity,
label-free, real-time detection (gas/liquid phase), chip renewable
BI-SPR
Five channels
High sensitivity and
rapid detection
Sophisticated
analytical modules and flexible combinations: gas phase chemistry,
electrochemistry
To provide users with a
variety of research tools, high quality data and the most reasonable price
Technology ability
Base Station | Light source | 670nm |
Detection speed | 4ms |
Incident angles |
40-47 Deg (gas) 67-81 Deg (liquid)
|
Baseline noise | <0.06RU RMS(0.01 mDeg RMS) |
Fluid Handling | Number of sample flow channels | 5channels |
Flow rate | 1.0 to 250 μL/min (application dependent) |
Sample injection volume | >50ul (application dependent) |
Solution
transfer method | Semi-automatic/full-automatic |
Minimum
injection time | <0.2s |
Molecular weight cutoff | 100 Da |
Analysis
Modules | 5
channels flow injection modules |
Life Science
Applications
Ø DNA
- protein,protein-protein,protein-drug reaction
Ø Gene Detection:interaction between DNA-DNA and such
as single nucleotide polymorphism
Ø Immunosensing: BI-SPR technology is useful for antibody - antigen, ligand
and receptor label-free real-time affinity and kinetic constant reactions.The
concentration and distribution of target sites can give important information.
Ø Conformational changes and folding of proteins:Conformational changes in proteins and other molecules tend to be very small
and rapid.The SPR technology of BI is able to be monitored, which can also
reveal the important role of proteins in some biological functions and
processes in the reference of small conformational changes in molecules
Ø Drug development:BI-SPR provides high quality data on
the dynamics, affinity and other binding properties in the process of drug
development.
Electrochemical
Application
SPR is used to detect
mass changes on the surface of metal films, while electrochemistry is used to
analyze electrochemical and kinetic processes of substances.If both of them can
be combined, their superiorities will be brought into full play. For example,
adsorption or bonding of biomacromolecules on metal films can be detected by
SPR Angle, while conformation changes or redox processes of biomacromolecules
at different potentials can be detected by electrochemical system.
Ø Electropolymerization, electrodeposition, corrosion process (polymer
secondary battery, etc.)
Ø Immunosense
Ø Conformational transformation of surface-fixed redox molecules
(conformation transformation of biological macromolecules or proteins)
Ø Potential controlled molecular adsorption and charge transfer processes
Food
environmental inspection
Chemical vapor
sense
Gas-solid interface research, toxic gas
molecular detection, clinical chemical analysis
Domestic users (in no
particular order)
Nanjing university
Southeast university
University of science
and technology of China
National center for
nanoscience
Tsinghua university
Ecological environment
center of Chinese Academy of Sciences
Jinan university
Shanghai health medical
college
Hunan agricultural
university
Central south
university
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Reference(part)
1. (English) Akshay Jain, Ashutosh Barve, Zhen Zhao, John Peter Fetse, Hao Liu, Yuanke Li, Kun Cheng, “Targeted Delivery of an siRNA/PNA Hybrid Nanocomplex Reverses Carbon Tetrachloride‐Induced Liver Fibrosis”, Advanced Therapeutics 2019, 1900046
2. (English) Qinghua Liu, Xiaoying Wang, Andrew Benedict, Lusine Janibekyan, Stephanie Wong Su, Yixian Wang, Feimeng Zhou, “Surface Plasmon Resonance Coupled with Potential‐step Chronoamperometry: Theory and Applications for Quantitative Measurements of Electrodeposited Thin Films” Electroanalysis, 2 Jul 2019, DOI: 10.1002/elan.201900006
3. (English) Ling Wu, Yuqing Hu, Yuhan He, Yonghong Xia, Hanwen Lu, Zhong Cao, Xinyao Yi and Jianxiu Wang, "Dual-Channel Surface Plasmon Resonance Monitoring of Intracellular Levels of p53-MDM2 Complex and Caspase-3 Induced by MDM2 Antagonist Nutlin-3", Analyst, 2019, DOI: 10.1039/C9AN00301K
4. (English) Zhongxiu Jiang, Baochai Shen and Juan Xiang, "Metal-dependent interactions of metallothionein-3 β-domain with amyloid-β peptide and related physiological implications", Journal of Inorganic Biochemistry, Volume 196, July 2019, 110693
5. (English) Zhenzhen Yin, Shuhui Wang, Baochai Shen, Chunyan Deng, Qiuyun Tu, Yan Jin, Lu Shen, Bin Jiao, and Juan Xiang, "Coimmunocapture and Electrochemical Quantitation of Total and Phosphorylated Amyloid-β40 Monomers", Anal. Chem. 2019, 91, 5, 3539-3545
6. (English) Xiaoying Wang, Patrycja Magdziarz, Ernest Enriquez, Wang Zhao, Chris Quan, Narek Darabedian, Jamil Momand and Feimeng Zhou, "Surface plasmon resonance and cytotoxicity assays of drug efficacies predicted computationally to inhibit p53/MDM2 interaction", Analytical Biochemistry Volume 569, 15 March 2019, Pages 53-58
7. (English) Ganesan Senthil Kumar, Meng S. Choy, Dorothy M. Koveal, Michael K. Lorinsky, Scott P. Lyons, Arminja N. Kettenbach, Rebecca Page, Wolfgang Peti, “Identification of the substrate recruitment mechanism of the muscle glycogen protein phosphatase 1 holoenzyme”, Science Advances, 2018, 4, eaau6044
8. (English) Fatemeh Ejeian, Parisa Etedali, Hajar-Alsadt, Mansouri-Tehrani, Asieh Soozanipour, Ze-Xian Low, Mohsen Asadnia, Asghar Taheri-Kafrani, and Amir Razmjou, "Biosensors for wastewater monitoring: A review", Biosensors and Bioelectronics, 118, 30 October 2018, 66-79
9. Xiaoying Wang and Feimeng Zhou, "Dual-Valve and Counter-Flow Surface Plasmon
Resonance" Anal. Chem., 2018, 90 (8), pp 4972–4977
10. Nan-Fu Chiu and Ting-Li Lin, "Affinity capture surface carboxyl-functionalized MoS2
sheets to enhance the sensitivity of surface plasmon resonance immunosensors" Talanta,
Volume 185, 1 August 2018, Pages 174-181
11. Nan-Fu Chiu, Ting-Li Lin, Chia-Tzu Kuo,"Highly sensitive carboxyl-graphene oxide-based